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Boster Bio Anti-TGN46 Rabbit Monoclonal Antibody catalog # M06591. Tested in WB, IHC, IP applications. This antibody reacts with Human.
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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
doi: 10.1083/jcb.200501162
Figure Lengend Snippet: The loss of optineurin results in Golgi fragmentation. Mock (a–c) or optineurin siRNA-treated HeLa cells (d–f and d′–f′) were double labeled in immunofluorescence experiments with TGN46 (b, e, and e′) and GM130 (a, d, and d′). White boxes indicate areas enlarged in the pictures below (d′, e′, and f′). The Golgi fragments contain both marker proteins as indicated by the yellow color in the merged images (c, f, and f′). The arrows highlight the overlap between the marker proteins in (d′–f′). Bars, 10 μm. TEM analysis of mock (g) or siRNA-transfected (h) HeLa cells. Arrows indicate the position of Golgi stacks that can be found in both mock-transfected and siRNA-transfected cells. Bars, 200 nm.
Article Snippet: The following antibodies were used: affinity-purified rabbit pAb to human full-length optineurin (α-Optn; GenBank/EMBLDDBJ accession no. AF061034 ); monoclonal α-VSV-G to the luminal domain (a gift from R. Pepperkok and J. Simpson, EMBL, Heidelberg, Germany); polyclonal α-optineurin (Cayman); monoclonal α-GM130 (BD Transduction Laboratories); monoclonal α-TGN38 ( ); monoclonal α-GFP (Qbiogene); polyclonal α-GFP (Molecular Probes); monoclonal,
Techniques: Labeling, Immunofluorescence, Marker, Transfection